Effects of blue®m against Streptococcus mutans biofilm and its virulence gene expression
A new recent study form Brasil showing that blue®m mouthwash has an antimicrobial capacity against Streptococcus mutans and is a more effective clinical alternative for CHX.
Published in the Brazilian Dental Journal 2023
Veronica Canela Estevam dos Santos 1), Patricia Milagros Maquera-Huacho 1), Maria Júlia Mancim Imbriani 1),2), Vivian M. Tellaroli Rodrigues Minhaco 1),2), Denise M. Palomari Spolidorio 1).
(1) Department of Physiology and Pathology, School of Dentistry, São Paulo State University (Unesp), Araraquara, SP, Brazil
(2) Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.
Abstract: This study evaluated the antimicrobial capacity of blue®m mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. blue®m showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed a significant effect of blue®m on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with blue®m at a concentration of 25%. Moreover, blue®m exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of blue®m against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of blue®m as an alternative agent for the control of oral biofilm.
Conclusion: The present study showed that blue®m has antibiofilm activity against S. mutans, presenting itself as a biocompatible product. This product has an antimicrobial effect against S. mutans, promoting bactericidal, bacteriostatic, antibiofilm, and non-cytotoxic effects at low concentrations. Moreover, blue®m is able to reduce the expression of the gbpA gene, interfering with the adhesion capacity of S. mutans. However, additional in vivo and in vitro tests are still needed to corroborate and elucidate its mechanism of action.