Metabolic activity of hydro-carbon-oxo-borate on a multispecies subgingival periodontal biofilm: a short communication

A recent publication in the Journal of Clinical and Medical Images by the Neodent team in Brazil, working at the Latin American Institute of Dental Research and Education (ILAPEO), highlights their innovative approach to post-operative care. ILAPEO is notably the first chlorhexidine-free university in the world. In this study, patients are recommended to use BlueM mouthwash after surgery. We are very proud that Dr. Geninho Thomé and his team recognize and value the advantages of BlueM products in enhancing patient care.

By Thomé G 1), Bernardes SR 1), Cartelli C2, Uhlendorf J 2) and Deliberador TM 1)*
Jamil Awad Shibli 1 & Thayane Furtado Rocha 1 & Fernanda Coelho 2 & Ticiana Sidorenko de Oliveira Capote 2 &
Sybele Saska 3 & Marcelo A. Melo 1 & João Marcos Spessoto Pingueiro 1 & Marcelo de Faveri 1 & Bruno Bueno-Silva 1

Abstract
Objective This study evaluated the metabolic activity of hydro-carbon-oxo-borate complex (HCOBc) on a multispecies subgingival biofilm as well as its effects on cytotoxicity. Materials and methods The subgingival biofilm with 32 species related to periodontitis was formed in the Calgary Biofilm Device (CBD) for 7 days. Two different therapeutic schemes were adopted: (1) treatment with HCOBc, 0.12% chlorhexidine (CHX), and negative control group (without treatment) from day 3 until day 6, two times a day for 1 min each time, totaling 8 treatments and (2) a 24-h treatment on a biofilm grown for 6 days. After 7 days of formation, biofilm metabolic activity was determined by colorimetry assay, and bacterial counts and proportions of complexes were determined by DNA DNA hybridi- zation. Both substances’ cytotoxicity was evaluated by cell viability (XTT assay) and clonogenic survival assay on ovary epithelial CHO-K1 cells and an osteoblast precursor from calvaria MC3T3-E1 cells.

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